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1.
The sound between scenes in Law and Order. The expression originated when 4chan changed the "Post Successful" page and font to look like the Law and Order black screen between scenes. The page played the sound, "Dun Dun," from Law and Order. 4chan /b/tards began to post the expression, "Dun Dun," anywhere to import a sense of finality or just randomly.
Tits or gtfo. Dun dun.
автор: Transmigraine 25 мая 2010
 
2.
someone who dun-dun dem all
dey call her dun-dun...dun-dun em all!!!
автор: Rae Nicole 28 мая 2008
 
3.
When a person can't get himself to talk about a certain ongoing subject (normally on chatrooms) and is put in a awkward situation, where some might expect a reaction from him and he goes,

"dun dun."

It comes from "so done with this". Usually related with Otaku culture for some reason.
Person 1: I just raped this bitch so fucking good, i swear i did.

Person 2: Hahaha so funny!

Person 3. o.o.. dun dun
автор: TheWanAndOnly 19 декабря 2013
 
4.
ur people. ur ace
Chill out dun dun
автор: DatKiDD 5 декабря 2003
 
5.
A mexician whine. Comes from "uh uh", but in spanish, and annoying.
Gordito: Hey, make me some tacos.
Jon: Dun dun!
автор: ZONE 13 марта 2005